Thermoresponsive Hydrogels as Microniches for Growth and Controlled Release of Induced Pluripotent Stem Cells

The recently emerging stem-cell artificial niche engineering in induced pluripotent stem cell (iPSCs) 3D cultures has provided enormous opportunities to fully utilize the potential of these cells biomedical applications. Although a chemically defined environment can supply with desirable safety for clinical use, establishing an degradable controlled release proliferated under mild conditions is still big challenge. Here, advanced releasable iPSC reported based on dendritic polyglycerol and poly(N-isopropylacrylamide)-co-polyethylene glycol polymers via physical–chemical cogelation strategy. Benefiting from synthetic materials their precise cooperation by covalent cross-linking physical phase transition, cogelation-based system be adjusted optimal parameters owns high biocompatibility support robust production quality iPSCs excellent expansion efficiency. Moreover, expanded released out controllably only adjusting temperature. Overall, this hydrogel scaffold shows great promise culture downstream